Design of optogenetic tools

Berndt A*, Lee SY*, Wietek J*, Ramakrishnan C, Steinberg EE, Rashid AJ, Kim H, Park S, Santoro A, Frankland PW, Iyer S, Pak S, Delp SL, Malenka RC, Josselyn S, Carlen M, Hegemann P, and Deisseroth K: Determinants of channelrhodopsin ion selectivity: structural principles for optogenetics. PNAS (2016)

Tee BC*, Chortos A*, Berndt A*, Nguyen AK, Tom A, McGuire A, Lin ZC, Tien K, Bae WG, Wang H, Mei P, Chou HH, Cui B, Deisseroth K, Ng TN, Bao Z: A skin-inspired organic digital mechanoreceptor. Science (2015)

Rajasethupathy P, Sankaran S, Marshel JH, Kim CK, Ferenczi E, Lee SY, Berndt A, Ramakrishnan C, Jaffe, Lo M, Liston C, Deisseroth K: Projections from neocortex mediate top-down control of memory retrieval. Nature (2015)

Berndt A*, Lee SY*, Ramakrishnan C and Deisseroth K: Structure-guided transformation of channelrhodopsin into a light-activated chloride channel. Science (2014)

Berndt A*, Schoenenberger P*, Mattis J, Tye KM, Deisseroth K, Hegemann P and Oertner TG: High efficiency channelrhodopsins for fast neuronal stimulation at low light levels. PNAS (2011)

Gunaydin LA*, Yizhar O*, Berndt A*, Sohal VS, Deisseroth K and Hegemann P: Ultrafast optogenetic control. Nature Neuroscience (2010)

Berndt A*, Yizhar O*, Gunaydin LA*, Hegemann P and Deisseroth K: Bi-stable neural state switches. Nature Neuroscience (2009)

molecular mechanism of light-activated cation channels

Ritter E, Stehfest K, Berndt A, Hegemann P and Bartl FJ: Monitoring light-induced structural changes of Channelrhodopsin-2 by UV-visible and Fourier transform infrared spectroscopy. The Journal of Biological Chemistry (2008)

Stehfest K, Ritter E, Berndt A, Bartl F and Hegemann P: The branched photocycle of the slow cycling channelrhodopsin-2 mutant C128T. Journal of Molecular Biology (2010)

Berndt A*, Prigge M*, Gradmann D and Hegemann P: Two open states with progressive proton selectivities in the branched channelrhodopsin-2 photocycle. Biophysical Journal (2010)

Gradmann D, Berndt A, Schneider F and Hegemann P: Rectification of the channelrhodopsin early conductance. Biophysical Journal (2011)

Eisenhauer K, Kuhne J, Ritter E, Berndt A, Wolf S, Freier E, Bartl F, Hegemann P, Gerwert K: In channelrhodopsin-2 Glu-90 is crucial for ion selectivity and is deprotonated during the
photocycle. The Journal of Biological Chemistry (2012)

Collaborative research projects

Iyer SM, Vesuna S, Ramakrishnan C, Huynh K, Young S, Berndt A, Lee SY, Gorini CJ, Deisseroth K, Delp SL: Optogenetic and chemogenetic strategies for sustained inhibition of pain. Scientific Reports (2016)

Ferenczi EA, Vierock J, Atsuta-Tsunoda K, Tsunoda SP, Ramakrishnan C, Gorini C, Thompson K, Lee SY, Berndt A, Perry C, Minniberger S, Vogt A, Mattis J, Prakash R, Delp S, Deisseroth K, Hegemann P: Optogenetic approaches addressing extracellular modulation of neural excitability. Scientific Reports (2016)

Kim CK, Yang SJ, Pichamoorthy N, Young NP, Kauvar I, Jennings JH, Lerner TN, Berndt A, Lee SY, Ramakrishnan C, Davidson TJ, Inoue M, Bito H, Deisseroth K: Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain. Nature Neuroscience (2016)

Kim CK, Miri A, Leung LC, Berndt A, Mourrain P, Tank DW, Burdine RD: Prolonged, brain-wide expression of nuclear-localized GCaMP3 for functional circuit mapping. Frontiers in Neural Circuits (2014)

Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ and Deisseroth K: INTRSECT: single-component targeting of cells using multiple-feature Boolean logic. Nature Methods (2014)

* co-first author